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Primer Design

PCR primer planning using human mispriming library:

 
Primer loops/dimers testing at oligo analyzer website
http://207.32.43.248/oligocalc.asp:
 
Primer melting temperature testing:
 
Testing for mispriming:

Database Work

Haploview

Hapmap

SNPs

SNAP_Proxy

NCBI:    dbSNP: Database of Single Nucleotide Polymorphisms

            Entrez Gene

            Human Map Viewer

UW-FHCRC Variation Discovery Resource

HGBASE: Human Genic Bi-Allelic Sequences Database

LeeLab

Gene Canvas

The SNP Consortium

University of Utah

 

Miscellaneous

UCLA Human Genetics

Protein Data Bank

BLAST server at NCBI

OMIM: Online Mendelian Inheritance in Man

Medical Genetics

National Center for Biotechnology Information (NCBI)

 

Human Genome

National Human Genome Research Institute

Human Genome Project Working Draft (UC Santa Cruz)

NCBI: Human Genome Resources

Genome: Ensembl

 

 

Dead links

http://playground.idtdna.com/program/oligocalc/oligocalc.asp