|
Primer Design
PCR primer planning using human mispriming library:
Primer loops/dimers
testing at oligo analyzer website
http://207.32.43.248/oligocalc.asp:
Primer melting
temperature testing:
Testing for
mispriming:
Database Work SNPs NCBI: dbSNP: Database of Single Nucleotide Polymorphisms UW-FHCRC Variation Discovery Resource HGBASE: Human Genic Bi-Allelic Sequences Database
Miscellaneous OMIM: Online Mendelian Inheritance in Man National Center for Biotechnology Information (NCBI)
Human Genome National Human Genome Research Institute Human Genome Project Working Draft (UC Santa Cruz) NCBI: Human Genome Resources
Dead links http://playground.idtdna.com/program/oligocalc/oligocalc.asp
|